種屬

人

別稱

SNF96.2; sNF96-2

組織來源

施萬細(xì)胞

疾病

1型神經(jīng)纖維瘤病Nf1

傳代比例/細(xì)胞消化

1:2傳代,

完全培養(yǎng)基配置

DMEM培養(yǎng)基 ；10%胎牛血清 ；1%雙抗

簡介

sNF96.2 is a Schwann cell line that was isolated in 1996 from a 28-year-old, male patient with neurofibromatosis type 1 Nf1.The sNF96.2 cell line was derived from a recurrent mass associated with nerve and diagnosed as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75.

形態(tài)

雪旺細(xì)胞樣

生長特征

貼壁生長

STR

Amelogenin X CSF1PO 12 D5S818 11 D7S820 10,11 D13S317 10 D16S539 11 TH01 6 TPOX 11

vWA 17,19

倍增時間

~33 h

培養(yǎng)條件

氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。

凍存條件

凍存液：90%FBS，DMSO 10%,

或使用非程序凍存液：官網(wǎng)貨號JY-H040

保藏機(jī)構(gòu)

ATCC; CRL-2884

產(chǎn)品使用

僅限于科學(xué)研究，不可作為動物或人類疾病的治療產(chǎn)品使用。